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Fingeraftryk af cardiolipin i leukocytter ved massespektrometri til hurtig diagnose af Barth syndrom
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Chapters
- 00:04Introduction
- 00:53Isolation of Leukocytes by Dextran Sedimentation of Red Blood Cells
- 02:57“Mini-Extraction” of Lipids from Isolated Leukocytes
- 04:03Lipid Analysis by MALDI-TOF/MS
- 05:00Results: Diagnosis of Barth Syndrome Using Leukocyte CL Fingerprints and (MLCL + CLi)/CLm Ratios
- 06:06Conclusion
Denne protokol viser, hvordan man opnår et massespektrometrisk "fingeraftryk" af leukocyt cardiolipin til diagnosticering af Barth syndrom. Vurderingen af forhøjet monolysocardiolipin til cardiolipin-forhold diskriminerer patienter med Barth syndrom fra patienter med kontrol hjertesvigt med 100% følsomhed og specificitet.
