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Biochemistry
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顺磁弛豫增强,用于检测和表征固有无序蛋白质的自缔合
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Biochemistry
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JoVE Journal
Biochemistry
Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins
Please note that all translations are automatically generated.
Click here for the English version.
顺磁弛豫增强,用于检测和表征固有无序蛋白质的自缔合
DOI:
10.3791/63057-v
•
07:24 min
•
September 23, 2021
•
Courtney N. Johnson
,
David S. Libich
1
Department of Biochemistry and Structural Biology and Greehey Children’s Cancer Research Institute
,
The University of Texas Health Science Center at San Antonio
Chapters
00:05
Introduction
00:49
Conjugating the 3-Maleimido-PROXYL Nitroxide Spin Label
01:38
Preparing the NMR Sample for Measuring Intramolecular PRE
02:11
Setting Up the NMR Spectrometer and Experiment Specific Parameters
03:51
Setting Up the
1
H
N
–
T
2
Experiment
04:58
Making a Diamagnetic Sample by Reducing Spin-Label with Ascorbic Acid
05:52
Results:
1
H
N
–
T
2
Rates for Residues 171 – 264 of the Intrinsically Disordered Domain of EWSR1
06:30
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
提出了一种应用顺磁弛豫增强核磁共振波谱来检测固有无序蛋白质中弱和瞬态分子间和分子内相互作用的方案。
Tags
Paramagnetic Relaxation Enhancement
Intrinsically Disordered Proteins
Biomolecular Interactions
NMR Techniques
Spin Label
15N Isotopically Labeled Protein
Gel Filtration
Ellman’s Reagent
NMR Sample Preparation
Pulse Calibration
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