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JoVE Journal
Biochemistry

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Use of Electron Paramagnetic Resonance in Biological Samples at Ambient Temperature and 77 K
 

Use of Electron Paramagnetic Resonance in Biological Samples at Ambient Temperature and 77 K

Article doi: 10.3791/58461
January 11th, 2019

Summary January 11th, 2019

Electron paramagnetic resonance (EPR) spectroscopy is an unambiguous method to measure free radicals. The use of selective spin probes allows for detection of free radicals in different cellular compartments. We present a practical, efficient method to collect biological samples that facilitate treating, storing, and transferring samples for EPR measurements.

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