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JoVE Journal Neuroscience
Flowcytometrisk analyse av multiple mitokondrielle parametere i humaninduserte pluripotente stamceller og deres nevrale og gliaderivater
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Flowcytometrisk analyse av multiple mitokondrielle parametere i humaninduserte pluripotente stamceller og deres nevrale og gliaderivater

Article DOI: 10.3791/63116-v • 06:09 min • November 8th, 2021
November 8th, 2021 •
*1,2, *1,2,3,4, 1,2, 1,2
1Department of Clinical Medicine (K1), University of Bergen, 2Neuro-SysMed, Center of Excellence for Clinical Research in Neurological Diseases, Haukeland University Hospital, 3Department of Neurosurgery, Qilu Hospital and Institute of Brain and Brain-Inspired Science, Cheeloo College of Medicine, Shandong University, 4Shandong Key Laboratory of Brain Function Remodeling
* These authors contributed equally

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Denne studien rapporterer en ny tilnærming til å måle flere mitokondrielle funksjonelle parametere basert på flowcytometri og dobbeltfarging med to fluorescerende reportere eller antistoffer for å oppdage endringer i mitokondrielt volum, mitokondrielt membranpotensial, reaktivt oksygenartsnivå, mitokondriell respiratorisk kjedesammensetning og mitokondrielt DNA.

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