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JoVE Journal Neuroscience
Flow cytometrisk analyse af flere mitokondrieparametre i humaninducerede pluripotente stamceller og deres neurale og gliale derivater
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Flow cytometrisk analyse af flere mitokondrieparametre i humaninducerede pluripotente stamceller og deres neurale og gliale derivater

Article DOI: 10.3791/63116-v • 06:09 min • November 8th, 2021
November 8th, 2021 •
*1,2, *1,2,3,4, 1,2, 1,2
1Department of Clinical Medicine (K1), University of Bergen, 2Neuro-SysMed, Center of Excellence for Clinical Research in Neurological Diseases, Haukeland University Hospital, 3Department of Neurosurgery, Qilu Hospital and Institute of Brain and Brain-Inspired Science, Cheeloo College of Medicine, Shandong University, 4Shandong Key Laboratory of Brain Function Remodeling
* These authors contributed equally

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Denne undersøgelse rapporterer en ny tilgang til måling af flere mitokondrielle funktionelle parametre baseret på flowcytometri og dobbeltfarvning med to fluorescerende reportere eller antistoffer til påvisning af ændringer i mitokondrievolumen, mitokondriemembranpotentiale, reaktivt iltartsniveau, mitokondrie respiratorisk kædesammensætning og mitokondrie-DNA.

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Neurovidenskab udgave 177
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