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Developmental Biology

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JoVE Journal Developmental Biology
Placa neural de Murine Alvo por In Utero Nano-Injection (NEPTUNE) no Dia Embrionário 7.5
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Placa neural de Murine Alvo por In Utero Nano-Injection (NEPTUNE) no Dia Embrionário 7.5

Article DOI: 10.3791/63148-v • 10:49 min • February 14th, 2022
February 14th, 2022 •
1, 1, 1, 1
1Department of Cell and Molecular Biology, Karolinska Institutet

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Neste protocolo, descrevemos como injetar a cavidade amniótica do camundongo no E7.5 com lentivírus, levando à transdução uniforme de toda a placa neural, com efeitos mínimos prejudiciais sobre a sobrevivência ou desenvolvimento embrionário.

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Biologia do Desenvolvimento Edição 180
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