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Bioengineering
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由去细胞化肺切片制备的工程肺组织
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
Engineered Lung Tissues Prepared from Decellularized Lung Slices
Please note that all translations are automatically generated.
Click here for the English version.
由去细胞化肺切片制备的工程肺组织
DOI:
10.3791/63151-v
•
08:01 min
•
January 21, 2022
•
Katherine L. Leiby
2
,
Ronald Ng
,
Stuart G. Campbell
3
,
Laura E. Niklason
4
1
Department of Biomedical Engineering
,
Yale University
,
2
Yale School of Medicine
,
3
Department of Cellular and Molecular Physiology
,
Yale School of Medicine
,
4
Department of Anesthesiology
,
Yale School of Medicine
Chapters
00:04
Introduction
00:35
Harvest of Rat Lungs
01:15
Cutting and Clipping the Lung Slices
02:25
Slice Decellularization
03:35
Slice Recellularization and Culture
06:20
Results: Visualization of the Structure of Decellularized Lung Scaffolds and Recellularized Engineered Lung Tissues (ELTs)
07:35
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了一种通过用肺泡上皮2型细胞,成纤维细胞和内皮细胞重新填充去细胞化精密切割的肺切片来产生可重复的小规模工程肺组织的方法。
Tags
Extracellular Matrix
Lung Slice
Decellularization
Cassette
Agarose
HBSS
PBS
Razor Blade
Orbital Shaker
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