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Konstrueret lungevæv fremstillet af decellulariserede lungeskiver
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Engineered Lung Tissues Prepared from Decellularized Lung Slices

Konstrueret lungevæv fremstillet af decellulariserede lungeskiver

DOI:
10.3791/63151-v

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08:01 min

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January 21, 2022

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, , ,
1Department of Biomedical Engineering,Yale University, 2Yale School of Medicine, 3Department of Cellular and Molecular Physiology,Yale School of Medicine, 4Department of Anesthesiology,Yale School of Medicine

Chapters

  • 00:04Introduction
  • 00:35Harvest of Rat Lungs
  • 01:15Cutting and Clipping the Lung Slices
  • 02:25Slice Decellularization
  • 03:35Slice Recellularization and Culture
  • 06:20Results: Visualization of the Structure of Decellularized Lung Scaffolds and Recellularized Engineered Lung Tissues (ELTs)
  • 07:35Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokol beskriver en metode til at generere reproducerbare, småskala konstruerede lungevæv ved at genbefolke decellulariserede præcisionsskårne lungeskiver med alveolære epiteltype 2-celler, fibroblaster og endotelceller.

Tags

Extracellular MatrixLung SliceDecellularizationCassetteAgaroseHBSSPBSRazor BladeOrbital Shaker

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