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Developmental Biology
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使用浸泡珠测定分析鸡胚胎发生过程中的细胞分化,形态发生和图案化
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JoVE Journal
Developmental Biology
Analysis of Cell Differentiation, Morphogenesis, and Patterning During Chicken Embryogenesis Using the Soaked-Bead Assay
Please note that all translations are automatically generated.
Click here for the English version.
使用浸泡珠测定分析鸡胚胎发生过程中的细胞分化,形态发生和图案化
DOI:
10.3791/63187-v
•
06:49 min
•
January 12, 2022
•
Jessica Cristina Marín-Llera
,
Jesús Chimal-Monroy
1
Departamento de Medicina Genómica y Toxicología Ambiental, Instituto de Investigaciones Biomédicas
,
Universidad Nacional Autónoma de México
Chapters
00:04
Introduction
00:48
Egg Incubation and Embryo Staging
02:08
Bead Preparation
04:34
Embryo Manipulation and Interdigit Implantation
05:42
Results: Evaluation of Cell Behavior in the Embryonic Chick Limb Using Soaked Beads
06:20
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
浸泡的磁珠测定涉及在任何发育时间点靶向递送测试试剂,以研究细胞分化和形态发生的调节。提出了适用于任何实验动物模型的详细方案,用于制备三种不同类型的浸泡珠子并将其植入鸡胚的间位中。
Tags
Cell Differentiation
Morphogenesis
Patterning
Chicken Embryogenesis
Soaked-bead Assay
Developmental Biology
Embryo
Hamburger-Hamilton Stages
Chorioallantoic Membrane
Affi-gel Heparin Beads
PBS
In Ovo
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