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使用浸泡珠测定分析鸡胚胎发生过程中的细胞分化,形态发生和图案化
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Analysis of Cell Differentiation, Morphogenesis, and Patterning During Chicken Embryogenesis Using the Soaked-Bead Assay
DOI:

06:49 min

January 12, 2022

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Chapters

  • 00:04Introduction
  • 00:48Egg Incubation and Embryo Staging
  • 02:08Bead Preparation
  • 04:34Embryo Manipulation and Interdigit Implantation
  • 05:42Results: Evaluation of Cell Behavior in the Embryonic Chick Limb Using Soaked Beads
  • 06:20Conclusion

Summary

Automatic Translation

浸泡的磁珠测定涉及在任何发育时间点靶向递送测试试剂,以研究细胞分化和形态发生的调节。提出了适用于任何实验动物模型的详细方案,用于制备三种不同类型的浸泡珠子并将其植入鸡胚的间位中。

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