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Journal / Biochemistry / The Application of Open Searching-based…
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Biochemistry

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JoVE Journal Biochemistry
The Application of Open Searching-based Approaches for the Identification of Acinetobacter baumannii O-linked Glycopeptides
Hydrolysis of a Ni-Schiff-Base Complex Using Conditions Suitable for Retention of Acid-labile Protecting Groups
Journal (Biochemistry)
Hydrolysis of a Ni-Schiff-Base Complex Using Conditions Suitable for Retention of Acid-labile Protecting Groups
A Spin-Tip Enrichment Strategy for Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues
Journal (Biochemistry)
A Spin-Tip Enrichment Strategy for Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues
 

The Application of Open Searching-based Approaches for the Identification of Acinetobacter baumannii O-linked Glycopeptides

Article DOI: 10.3791/63242-v • 08:37 min • November 2nd, 2021
November 2nd, 2021 •
*1, *1, *1, 1
1Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne
* These authors contributed equally

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Summary
Automatic Translation

Open searching enables the identification of glycopeptides decorated with previously unknown glycan compositions. Within this article, a streamlined approach for undertaking open searching and subsequent glycan-focused glycopeptide searches are presented for bacterial samples using Acinetobacter baumannii as a model.

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Open Searching-based Approaches Identification Acinetobacter Baumannii O-linked Glycopeptides Bacterial Glycoproteins Glycosylation Events Glycans Proteome Samples STB-RPS Stop-and-go Extraction Tips Peptide Binding SDB-RPS Stage Tips Washing Equilibration
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