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JoVE Journal
Biochemistry

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A Spin-Tip Enrichment Strategy for Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues
 

A Spin-Tip Enrichment Strategy for Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues

Article DOI: 10.3791/63735-v 09:16 min May 4th, 2022
May 4th, 2022

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Summary

Post-translational modifications (PTMs) change protein structures and functions. Methods for the simultaneous enrichment of multiple PTM types can maximize coverage in analyses. We present a protocol using dual-functional Ti(IV)-immobilized metal affinity chromatography followed by mass spectrometry for the simultaneous enrichment and analysis of protein N-glycosylation and phosphorylation in pancreatic tissues.

Tags

Spin-Tip Enrichment Strategy N-glycopeptides Phosphopeptides Human Pancreatic Tissues Titanium Immobilized Metal Affinity Chromatography Biomolecular Information Separation Mechanisms Mass Spectrometry Analysis Glycosylation Phosphorylation Disease Biomarker Discovery Diabetes Cancer Spin Tips Centrifugation Particulates Tissue Pulverizer
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