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Experimental and Data Analysis Workflow for Soft Matter Nanoindentation
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Bioengineering
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JoVE Journal Bioengineering
Experimental and Data Analysis Workflow for Soft Matter Nanoindentation

Experimental and Data Analysis Workflow for Soft Matter Nanoindentation

DOI:
10.3791/63401-v

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13:04 min

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January 18, 2022

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, , , , ,
1Centre for the Cellular Microenvironment, James Watt School of Engineering,University of Glasgow, 2Optics 11 life, 3Laboratory of Biosensors and Bioelectronics,ETH Zürich

Chapters

  • 00:05Introduction
  • 00:43Probe Calibration
  • 03:38Measuring the Young s Modulus of Soft Materials
  • 05:30Data Analysis
  • 06:33Formal Data Analysis
  • 10:08Results: Acquiring Nanoindentation Data on Hydrogels and Cells
  • 12:26Conclusion

Summary

Automatic Translation

Automatic Translation

The protocol presents a complete workflow for soft material nanoindentation experiments, including hydrogels and cells. First, the experimental steps to acquire force spectroscopy data are detailed; then, the analysis of such data is detailed through a newly developed open-source Python software, which is free to download from GitHub.

Tags

Keywords: NanoindentationHydrogelsCellsAtomic Force MicroscopySoft MatterMechanical PropertiesCalibrationInterferometerSurface DetectionData Analysis

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