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Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood
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1Department of Biomedical Engineering,School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), UNIST-gil 50, Ulsan, Republic of Korea, 2Agency for Defense Development (ADD), Daejeon, Republic of Korea, 3KOGAS (Korea Gas Corporation) Research Institute, 4Center for Soft and Living Matter,Institute for Basic Science (IBS)
Chapters
- 00:00Title
- 01:22Introduction
- 01:47Fabrication of TiO2 NF Mats and Discs
- 02:35Integration of TiO2 NF Mat into a Centrifugal Microfluidic Device
- 04:06Surface Modification and Disc Assembly
- 05:35On-disc Immunoassay and Detection
- 07:11Results
- 08:27Conclusion
This protocol demonstrates how to achieve femto molar detection sensitivity of proteins in 10 µL of whole blood within 30 min. This can be achieved by using electrospun nanofibrous mats integrated in a lab-on-a-disc, which offers high surface area as well as effective mixing and washing for enhanced signal-to-noise ratio.
