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JoVE Journal
Bioengineering

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Lignin Down-regulation of Zea mays via dsRNAi and Klason Lignin Analysis
 

Lignin Down-regulation of Zea mays via dsRNAi and Klason Lignin Analysis

Article DOI: 10.3791/51340
July 23rd, 2014

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Summary July 23rd, 2014

A double stranded RNA interference (dsRNAi) technique is employed to down-regulate the maize cinnamoyl coenzyme A reductase (ZmCCR1) gene to lower plant lignin content. Lignin down-regulation from the cell wall is visualized by microscopic analyses and quantified by the Klason method. Compositional changes in hemicellulose and crystalline cellulose are analyzed.

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