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Bioengineering
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通过表面等离子体共振
设计
抗病毒药物
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Engineering Antiviral Agents
via
Surface Plasmon Resonance
Please note that all translations are automatically generated.
Click here for the English version.
通过表面等离子体共振
设计
抗病毒药物
DOI:
10.3791/63541-v
•
13:00 min
•
June 14, 2022
•
Irene Maier
2
1
Department of Environmental Health Sciences
,
University of California, Los Angeles
,
2
Department of General Surgery
,
Medical University of Vienna
Chapters
00:05
Introduction
01:05
Preparation of LB-Agar Plates with Plasmid DNA Transformed Cells and Site-Directed Mutagenesis
01:52
Transformation of Bacterial Cells
03:12
Expression and Protein Purification
06:22
SPR Spectroscopy
07:17
SPR Binding Assay for Cyanovirin-N Binding to Hemagglutinin, S Protein, and RBD
09:56
Results: SPR Binding Assays to Examine Cyanovirin-N Binding to Hemagglutinin and Mannosylated Peptides
11:55
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本协议描述了用于SPR结合测定的新工具,以检查CV-N与HA,S糖蛋白,相关杂交型聚糖和高甘露糖寡糖的结合。SPR用于测定将二聚体或单体CV-N与这些聚糖结合的K
D
。
Tags
Surface Plasmon Resonance
SPR
Protein-ligand Interactions
Virus Binding
Kinetic Analysis
Drug Development
Antibody Characterization
DNA Template
DpnI Digestion
Competent Cells
Transformation
Seed Culture
Expression Culture
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