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通过表面等离子体共振 设计 抗病毒药物
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Engineering Antiviral Agents via Surface Plasmon Resonance
DOI:

13:00 min

June 14, 2022

Chapters

  • 00:05Introduction
  • 01:05Preparation of LB-Agar Plates with Plasmid DNA Transformed Cells and Site-Directed Mutagenesis
  • 01:52Transformation of Bacterial Cells
  • 03:12Expression and Protein Purification
  • 06:22SPR Spectroscopy
  • 07:17SPR Binding Assay for Cyanovirin-N Binding to Hemagglutinin, S Protein, and RBD
  • 09:56Results: SPR Binding Assays to Examine Cyanovirin-N Binding to Hemagglutinin and Mannosylated Peptides
  • 11:55Conclusion

Summary

Automatic Translation

本协议描述了用于SPR结合测定的新工具,以检查CV-N与HA,S糖蛋白,相关杂交型聚糖和高甘露糖寡糖的结合。SPR用于测定将二聚体或单体CV-N与这些聚糖结合的KD

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