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Subtype-selective Electroporation of Cortical Interneurons
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Neuroscience
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JoVE Journal Neuroscience
Subtype-selective Electroporation of Cortical Interneurons

Subtype-selective Electroporation of Cortical Interneurons

DOI:
10.3791/51518-v

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06:42 min

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August 18, 2014

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,
1NYU Neuroscience Institute,New York University School of Medicine, 2Brain and Mind Research Institute,Weill Cornell Medical College

Chapters

  • 00:05Title
  • 01:19Surgery
  • 02:38Electroporation
  • 05:10Results: Subtype-selective Electroporation of Cortical Interneurons
  • 05:56Conclusion

Summary

Automatic Translation

Automatic Translation

This procedure shows how to target interneurons in the developing mouse forebrain by means of in utero electroporation. This technique was particularly efficient to achieve selective gene expression in interneuron subtypes destined to the superficial layers of the cortex.

Tags

Keywords: Cortical InterneuronsSubtype-selectiveElectroporationGABAergic InterneuronsDlx5/Dlx6 EnhancerIn Utero ElectroporationCentral Nervous SystemGenetic TargetingNeuronal Diversity

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