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テンソメトリック小容量チャンバーミオグラフィを用いたマウス胸部大動脈の内皮依存性血管弛緩の測定
JoVE Journal
Biology
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JoVE Journal Biology
Measurement of Endothelium-Dependent Vasorelaxation in the Mouse Thoracic Aorta Using Tensometric Small Volume Chamber Myography
DOI:

09:47 min

August 12, 2022

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Chapters

  • 00:05Introduction
  • 00:56Myograph Unit Preparation
  • 02:03Isolation and Mounting of the Aortic Segments onto the Myograph Chamber
  • 03:24Measurement of Endothelium-Dependent Vasorelaxation in Aortic Rings
  • 05:18Effects of General Inhibitors of NO Production on Endothelium-Mediated Vasorelaxation
  • 06:25Contribution of the Endothelial Layer to Aortic Vasorelaxation
  • 08:00Results: Mechanisms Underlying Endothelial-Dependent Vasorelaxation Using Tensometric Small Volume Chamber Myography in a Murine Model
  • 09:06Conclusion

Summary

Automatic Translation

本プロトコルは、マウス大動脈内皮機能の実験的 ex vivo 評価におけるマルチチャンバーミオグラフシステムを用いたテンソメトリックミオグラフ技術の概念および技術的適用を記載する。

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