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テンソメトリック小容量チャンバーミオグラフィを用いたマウス胸部大動脈の内皮依存性血管弛緩の測定
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JoVE Journal
Biology
Measurement of Endothelium-Dependent Vasorelaxation in the Mouse Thoracic Aorta Using Tensometric Small Volume Chamber Myography
Please note that all translations are automatically generated.
Click here for the English version.
テンソメトリック小容量チャンバーミオグラフィを用いたマウス胸部大動脈の内皮依存性血管弛緩の測定
DOI:
10.3791/63918-v
•
09:47 min
•
August 12, 2022
•
Brikena Gusek
,
Robert Folk
,
Tala Curry
,
Mitra Esfandiarei
1
Department of Biomedical Sciences, College of Graduate Studies
,
Midwestern University
,
2
Department of Child Health, College of Medicine-Phoenix
,
University of Arizona, Phoenix
Chapters
00:05
Introduction
00:56
Myograph Unit Preparation
02:03
Isolation and Mounting of the Aortic Segments onto the Myograph Chamber
03:24
Measurement of Endothelium-Dependent Vasorelaxation in Aortic Rings
05:18
Effects of General Inhibitors of NO Production on Endothelium-Mediated Vasorelaxation
06:25
Contribution of the Endothelial Layer to Aortic Vasorelaxation
08:00
Results: Mechanisms Underlying Endothelial-Dependent Vasorelaxation Using Tensometric Small Volume Chamber Myography in a Murine Model
09:06
Conclusion
Summary
Automatic Translation
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Automatic Translation
本プロトコルは、マウス大動脈内皮機能の実験的
ex vivo
評価におけるマルチチャンバーミオグラフシステムを用いたテンソメトリックミオグラフ技術の概念および技術的適用を記載する。
Tags
Keywords: Tensometric Myography
Endothelium-dependent Vasorelaxation
Mouse Thoracic Aorta
HEPES-PSS Solution
High-potassium Solution
Carbogen Gas
Aortic Dissection
Aortic Segments
Myograph Unit
Data Acquisition
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