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Journal
/
Behavior
/
达乌尔
蝴蝶
幼虫的大规模趋重试验
JoVE Journal
Behavior
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JoVE Journal
Behavior
Large-Scale Gravitaxis Assay of
Caenorhabditis
Dauer Larvae
Please note that all translations are automatically generated.
Click here for the English version.
达乌尔
蝴蝶
幼虫的大规模趋重试验
DOI:
10.3791/64062-v
•
07:53 min
•
May 31, 2022
•
Caroline Ackley
,
Lindsey Washiashi
,
Ruchira Krishnamurthy
,
Joel H. Rothman
1
Molecular Cellular and Developmental Biology
,
University of California
Chapters
00:05
Introduction
00:47
Chamber Preparation
01:37
Filling the Chambers with Agar
02:33
Isolating Dauer Larvae
04:01
Adding Dauers to the Chamber
05:03
Running and Scoring the Assay
06:38
Results: Comparison of Gravitaxis Behavior Across Species
07:14
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本协议概述了用
Caenorhabditis
dauer幼虫进行大规模重力测定的方法。与基于平板的测定相比,该协议可以更好地检测重症行为。
Tags
Keywords: Gravitaxis Assay
Caenorhabditis Dauer Larvae
High Throughput
Gravity Sensation
Vestibular System
NGM Agar
Serological Pipettes
Bunsen Burner
Razor Blade
Pliers
Tweezers
Plastic Cutting Surface
Fume Hood
OP50 Bacteria
M9 Buffer
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