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Dissekering af celle-autonom funktion af skrøbeligt X mentalt retarderingsprotein i et auditivt kredsløb ved In Ovo-elektroporation
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Dissecting Cell-Autonomous Function of Fragile X Mental Retardation Protein in an Auditory Circuit by In Ovo Electroporation
DOI:

11:10 min

July 06, 2022

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Chapters

  • 00:05Introduction
  • 00:50Egg Preparation
  • 02:30Egg Windowing and Embryo Identification
  • 03:39Neural Tube Injection and Electroporation
  • 06:20Otocyst Injection and Electroporation
  • 07:44Results: FMRP Knockdown in the Chicken NM and Auditory Duct and Axonal Tracking of Transfected AG Neurons in the Brainstem
  • 10:18Conclusion

Summary

Automatic Translation

Ved hjælp af ovo elektroporation udtænkte vi en metode til selektivt at transficere det auditive indre øre og cochlearkernen i kyllingembryoner for at opnå en cellegruppespecifik knockdown af skrøbeligt X mentalt retarderingsprotein i diskrete perioder med kredsløbssamling.

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