JoVE Journal
Developmental Biology
Developmental Biology
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
Summary
Here, we describe a simple technique intended for the efficient generation of genetically modified mice called CRISPR RNP Electroporation of Zygotes (CRISPR-EZ). This method delivers editing reagents by electroporation into embryos at an efficiency approaching 100%. This protocol is effective for point mutations, small genomic insertions, and deletions in mammalian embryos.