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JoVE Journal Neuroscience
Human Microglia-like Cells
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Human Microglia-like Cells: Differentiation from Induced Pluripotent Stem Cells and In Vitro Live-cell Phagocytosis Assay using Human Synaptosomes

Article DOI: 10.3791/64323-v • 11:19 min • August 18th, 2022
August 18th, 2022 •
1,2, 1
1Department of Neurology, University of Massachusetts Chan Medical School, 2Translation Science Program, Morningside Graduate School of Biomedical Sciences, University of Massachusetts Chan Medical School

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Summary
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This protocol describes the differentiation process of human induced pluripotent stem cells (iPSCs) into microglia-like cells for in vitro experimentation. We also include a detailed procedure for generating human synaptosomes from iPSC-derived lower motor neurons that can be used as a substrate for in vitro phagocytosis assays using live-cell imaging systems.

Tags

Human Microglia-like Cells Induced Pluripotent Stem Cells (iPSCs) In Vitro Experimentation Microglia Differentiation Protocol Growth Factors High Purity And Yield Fully Humanized Phagocytosis Assay Media Evaporation Cell Culture Plate Survival Improvement Dissociation Reagent Single Cell Suspension Centrifuge Embryoid Body (EB) Medium Trypan Blue Cell Count
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