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Journal / Neuroscience / İnsan Mikroglia Benzeri Hücreler: İnsan…
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JoVE Journal Neuroscience
İnsan Mikroglia Benzeri Hücreler
High-density EEG Recordings of the Freely Moving Mice using Polyimide-based Microelectrode
Journal (Neuroscience)
High-density EEG Recordings of the Freely Moving Mice using Polyimide-based Microelectrode
Maintenance of a Lateral Fluid Percussion Injury Device
Journal (Neuroscience)
Maintenance of a Lateral Fluid Percussion Injury Device
 
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İnsan Mikroglia Benzeri Hücreler: İnsan Sinaptozomları Kullanılarak İndüklenmiş Pluripotent Kök Hücrelerden ve In Vitro Canlı Hücre Fagositoz Analizinden Farklılaşma

Article DOI: 10.3791/64323-v • 11:19 min • August 18th, 2022
August 18th, 2022 •
1,2, 1
1Department of Neurology, University of Massachusetts Chan Medical School, 2Translation Science Program, Morningside Graduate School of Biomedical Sciences, University of Massachusetts Chan Medical School

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Bu protokol, insan kaynaklı pluripotent kök hücrelerin (iPSC'ler) in vitro deney için mikroglia benzeri hücrelere farklılaşma sürecini açıklar. Ayrıca, canlı hücre görüntüleme sistemlerini kullanarak in vitro fagositoz testleri için bir substrat olarak kullanılabilecek iPSC kaynaklı alt motor nöronlardan insan sinaptozomları üretmek için ayrıntılı bir prosedür de sunuyoruz.

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