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Journal / Immunology and Infection / 流式细胞术 检测…
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Immunology and Infection

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JoVE Journal Immunology and Infection
流式细胞术 检测 接种乙型脑炎疫苗的儿童多功能T细胞
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流式细胞术 检测 接种乙型脑炎疫苗的儿童多功能T细胞

Article DOI: 10.3791/64671-v • 09:37 min • September 23rd, 2022
September 23rd, 2022 •
*1,2, *1,2,3, 1,2, 1,2, 1,2, 4, 1,2, 1,2
1Beijing Key Laboratory of Pediatric Respiratory Infectious Diseases, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, Laboratory of Infection and Virology, Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, 2Research Unit of Critical Infection in Children, 2019RU016, Chinese Academy of Medical Sciences, 3Department of Pediatric Rehabilitation, Beijing Boai Hospital, School of Rehabilitation Medicine, Capital Medical University, China Rehabilitation Research Center, 4Center of Excellence (Beijing), Becton Dickinson Medical Devices (Shanghai) Co Ltd
* These authors contributed equally

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Summary
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本协议结合了 离体 刺激和流式细胞术，以分析日本脑炎病毒（JEV）疫苗接种的儿童中外周血单核细胞（PBMC）中的多功能T细胞（TPF）谱。对JEV特异性TPF的检测方法和流式细胞术配色方案进行了测试，为类似研究提供参考。

Tags

免疫学与感染，第187期，多功能T细胞，日本脑炎，疫苗接种，流式细胞术
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