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Generation of Human Blood Vessel Organoids from Pluripotent Stem Cells
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Generation of Human Blood Vessel Organoids from Pluripotent Stem Cells

Generation of Human Blood Vessel Organoids from Pluripotent Stem Cells

DOI:
10.3791/64715-v

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09:46 min

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January 20, 2023

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,
1School of Biomedical Engineering,University of British Columbia, 2Life Sciences Institute,University of British Columbia, 3Department of Medical Genetics,University of British Columbia, 4Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA)

Chapters

  • 00:04Introduction
  • 00:52Day 0: Generation of Pluripotent Aggregates from Single-Cell Suspension
  • 02:34Day 1: Mesoderm Induction of the Aggregates
  • 03:41Day 4: Vascular Induction and Priming of the Aggregates
  • 04:20Day 6: Aggregate Embedding and Vessel Sprout Induction
  • 06:02Day 11: Isolation and Maturation of the Blood Vessel Organoids (BVOs)
  • 07:25Results: Stepwise Progression of Human Blood Vessel Organoid Generation from hPSCs
  • 09:13Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes the generation of self-organizing blood vessels from human pluripotent and induced pluripotent stem cells. These blood vessel networks exhibit an extensive and connected endothelial network surrounded by pericytes, smooth muscle actin, and a continuous basement membrane.

Tags

Blood Vessel OrganoidsPluripotent Stem CellsVasculogenesisAngiogenesisVascular DiseaseVascular PathologiesReproducibilityHigh ThroughputStem Cell LinesDiabetic VasculopathyAggregatesCell DissociationAggregation MediumSingle-cell SuspensionCell ViabilityMesoderm Media

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