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パルミチン酸誘発
In vitro
モデルにおける非アルコール性脂肪性肝疾患に対するプラチコジンDの保護効果の調査
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JoVE Journal
Medicine
Investigating the Protective Effects of Platycodin D on Non-Alcoholic Fatty Liver Disease in a Palmitic Acid-Induced
In Vitro
Model
Please note that all translations are automatically generated.
Click here for the English version.
パルミチン酸誘発
In vitro
モデルにおける非アルコール性脂肪性肝疾患に対するプラチコジンDの保護効果の調査
DOI:
10.3791/64816-v
•
08:20 min
•
December 02, 2022
•
Xingjian Wen*
1,2
,
Jia Wang*
1
,
Jiuyu Fan
,
Rui Chu
,
Yilong Chen
,
Yajing Xing
,
Na Li
2
,
Guixue Wang
1
Chongqing Academy of Chinese Materia Medica
,
2
Bioengineering College of Chongqing University
Chapters
00:05
Introduction
00:58
Pretreatment of AML-12 Cells for In Vitro NAFLD Model
02:32
Measuring the Change in ROS Production and Mitochondrial Membrane Potential
04:05
Measuring the Protein Expression Levels of LC3-II/LC3-I and p62/SQSTM1
05:59
Results: Effects of Platycodin D on Intracellular ROS, MMP Depolarization, and Protein Expression Levels in AML-12 Cells
07:49
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
このプロトコルは、パルミチン酸誘発
インビトロ
モデルにおける非アルコール性脂肪肝疾患に対するプラチコジンDの保護効果を調査します。
Tags
AML-12 Cells
Non-alcoholic Fatty Liver Disease (NAFLD)
Platycodin D
Palmitic Acid
Cell Model
In Vitro
Oxidative Stress
Mitochondrial Membrane Potential
Microscopy
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