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Floral-dip Transformation of Arabidopsis thaliana to Examine pTSO2::β-glucuronidase Reporter Gene Expression
JoVE Journal
Biology
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JoVE Journal Biology
Floral-dip Transformation of Arabidopsis thaliana to Examine pTSO2::β-glucuronidase Reporter Gene Expression
DOI:

10:24 min

June 11, 2010

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Chapters

  • 00:00Title
  • 01:00Introduction
  • 01:35Floral-dip Transformation of Arabidopsis thaliana
  • 05:20Selecting for Transgenic Plants
  • 07:25Examining pTS02::GUS Expression Patterns
  • 09:26Representative Images of GUS Expression in Arabidopsis thaliana Transformants
  • 09:57Conclusion

Summary

Automatic Translation

This article illustrates the floral-dip method of Agrobacterium tumefaciens -mediated transformation of Arabidopsis thaliana. By introducing a cell-cycle regulated promoter-reporter, pTSO2::β-glucuronidase (GUS), into Arabidopsis, we illustrates how one detects GUS reporter expression in transgenic seedlings.

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