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Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
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Biology
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Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification

DOI:
10.3791/3060-v

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10:21 min

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September 21, 2011

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, ,
1College of Nursing, Interdisciplinary Life Sciences Research Laboratory,Seattle University, 2College of Science and Engineering, Interdisciplinary Life Sciences Research Laboratory,Seattle University

Chapters

  • 00:05Title
  • 01:30Buffer and Sample Preparation
  • 02:48Physical Setup and Plumbing of the DuoFlow Chromatography System
  • 04:11Priming System Lines,Programming Run Method, and Equilibrating HIC Columns
  • 05:43Sample Loading, Programming the Software, and Running the Column Scouting Protocol
  • 07:36Representative Chromatogram and Fluorescent Fraction Data
  • 09:23Conclusion

Summary

Automatic Translation

Automatic Translation

An automated method for identifying suitable hydrophobic interaction chromatography (HIC) media to be used in the process of protein purification is presented. The method utilizes a medium-pressure liquid chromatography system including automated buffer blending, dynamic sample loop injection, sequential column selection, multi-wavelength analysis, and split fraction eluate collection.

Tags

Automated Hydrophobic Interaction ChromatographyProtein PurificationChromatographic Medium SelectionScoutingHydrophobicityAffinity ChromatographyIon Exchange ChromatographyProtein ConcentrationAmmonium SulfatePurification MethodNonpolar LigandChromatography ColumnSalt Concentration

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