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Chapters
- 00:05Title
- 01:17Preparation of a Cysteine Charged Sulfolink Resin
- 02:36Chromatographic Purification of Ribosomes Using Cysteine Charged Sulfolink Resin
- 05:18Puromycin Treatment & Purification of Ribosomes by Sedimentation Through Glycerol Cushions
- 07:28Representative Purification of Highly Active Yeast Ribosomes
- 08:48Conclusion
Contamination of preparations of eukaryotic ribosomes purified by traditional methods by co-purifying nucleases and proteases negatively impacts on downstream biochemical and structural analyses. A rapid and simple chromatographic purification method is used to solve this problem using yeast ribosomes as a model system.
Tags
Chromatographic PurificationYeast RibosomesLabile RibosomesProteasesNucleasesDifferential UltracentrifugationStructural IntegrityFunctionalityCysteine Charged Sulfolink ResinCo-purifying Proteolytic And Nucleolytic ActivitiesIntact RibosomesBiochemically Active RibosomesMammalian RibosomesTechnical Advancement
