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Changing the Direction and Orientation of Electric Field During Electric Pulses Application Improves Plasmid Gene Transfer in vitro
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JoVE Journal Medicine
Changing the Direction and Orientation of Electric Field During Electric Pulses Application Improves Plasmid Gene Transfer in vitro

Changing the Direction and Orientation of Electric Field During Electric Pulses Application Improves Plasmid Gene Transfer in vitro

DOI:
10.3791/3309-v

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04:46 min

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September 12, 2011

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, , , ,
1Department of Fundamentals of Electrical Engineering, Mathematics and Physics,University of Ljubljana, 2Department of Biomedical Engineering,University of Ljubljana

Chapters

  • 00:01Title
  • 00:04Introduction
  • 00:58Cell Culture Preparation for the Experiment
  • 01:42Hardware Equipment
  • 02:09Gene Electrotransfer Protocol
  • 03:31Image Acquisition and Determination of Gene Electrotransfer Efficacy

Summary

Automatic Translation

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Gene transfection by electroporation is improved approximately two times when orientation of electric field is changed during pulse application, while cell viability is not affected. The increase in gene transfection is caused by the increase of the membrane area which is made competent for DNA entry into the cell.

Tags

Electric FieldGene TransferPlasmid GeneIn VitroGene ElectrotransferCell MembraneDNA EntryViral VectorsElectric Pulse ProtocolsMembrane AreaGene TransfectionGreen Fluorescent Protein

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