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Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET
JoVE Journal
Biology
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JoVE Journal Biology
Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET
DOI:

13:38 min

January 27, 2012

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Chapters

  • 00:05Title
  • 02:19Introducing the Probe to Cells
  • 03:27Monitoring Ratiometric FRET
  • 06:12Verifying and Optimizing Conditions
  • 06:43Analyzing FRET
  • 11:24Representative Imaging Results
  • 12:52Conclusion

Summary

Automatic Translation

FRET-based reporters are increasingly used to monitor kinase and phosphatase activities in live cells. Here we describe a method on how to use FRET-based reporters to assess cell cycle-dependent changes in target phosphorylation.

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