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Preparation of an Awake Mouse for Recording Neural Responses and Injecting Tracers
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Preparation of an Awake Mouse for Recording Neural Responses and Injecting Tracers
DOI:

14:06 min

June 26, 2012

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Chapters

  • 00:05Title
  • 01:54Preparation of the Head Restraint
  • 04:14Stereotaxic Alignment for Craniotomy
  • 08:24Head-post and Ground Pin Installation
  • 10:20Craniotomy
  • 11:54Results: Electrophysiological Recordings and Tracing Studies of the Inferior Colliculus
  • 13:19Conclusion

Summary

Automatic Translation

Electrophysiological characterization of neuronal responses is important for understanding brain function and for guiding the placement of dyes for pathway tracing. However, many studies are performed in anesthetized animals. To understand brain function without anesthetics, we developed a method to record neuronal response properties and inject dyes in awake mouse.

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