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Preparation of an Awake Mouse for Recording Neural Responses and Injecting Tracers
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Neuroscience
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JoVE Journal Neuroscience
Preparation of an Awake Mouse for Recording Neural Responses and Injecting Tracers

Preparation of an Awake Mouse for Recording Neural Responses and Injecting Tracers

DOI:
10.3791/3755-v

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14:06 min

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June 26, 2012

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, , ,
1Department of Neuroscience,Johns Hopkins University, 2Garvan Institute of Medical Research, 3School of Biological Sciences,Washington State University, 4Department of Otolaryngology-HNS,Johns Hopkins University

Chapters

  • 00:05Title
  • 01:54Preparation of the Head Restraint
  • 04:14Stereotaxic Alignment for Craniotomy
  • 08:24Head-post and Ground Pin Installation
  • 10:20Craniotomy
  • 11:54Results: Electrophysiological Recordings and Tracing Studies of the Inferior Colliculus
  • 13:19Conclusion

Summary

Automatic Translation

Automatic Translation

Electrophysiological characterization of neuronal responses is important for understanding brain function and for guiding the placement of dyes for pathway tracing. However, many studies are performed in anesthetized animals. To understand brain function without anesthetics, we developed a method to record neuronal response properties and inject dyes in awake mouse.

Tags

Awake MouseRecording Neural ResponsesInjecting TracersAnesthesiaBrainstem NeuronsFundamental Response PropertiesDecerebrate PreparationElectrode ArraysChronic RecordingAwake And Behaving AnimalsTracer DyesHead Restraint Technique

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