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Chapters
- 00:05Title
- 01:50Endothelial Colony Forming Cells (ECFC) Outgrowth, Cloning, and Expansion
- 04:12In vitro Phenotypic Characterization of ECFC
- 10:58Results: Representative Phenotypic and Functional Analyses of ECFC Derived from Human Cord Blood
- 13:02Conclusion
Endothelial colony forming cells (ECFCs) are circulating endothelial cells with robust clonal proliferative potential that display intrinsic in vivo vessel forming ability. Phenotypic and functional characterization of outgrowth endothelial cells derived from CB are important to identify and isolate bona fide ECFCs for potential clinical application in repairing damaged tissues.
Tags
Phenotypic CharacterizationFunctional CharacterizationEndothelial Colony Forming CellsHuman Umbilical Cord BloodAngiogenesisVasculogenesisArteriogenesisCirculating Endothelial Progenitor CellsVascular RegenerationVascular RepairParacrine StimulationEPC MarkerProangiogenic Hematopoietic Stem CellsCirculating Angiogenic CellsTie2+ MonocytesMyeloid Progenitor CellsTumor Associated MacrophagesM2 Activated Macrophages
