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Neuroscience

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JoVE Journal Neuroscience
Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain
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Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain

Article DOI: 10.3791/3938-v • 13:11 min • July 12th, 2012
July 12th, 2012 •
1, 1, 1, 1
1Max Planck Institute of Psychiatry

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Summary
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A streamlined workflow to study DNA methylation and gene expression changes upon early-life stress is shown. Starting from maternal separation of newborn mice and isolation of discrete brain tissues, we represent a protocol to simultaneously isolate DNA and RNA from brain tissue punches for subsequent bisulfite sequencing and RT-PCR analysis.

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DNA Methylation Gene Expression Brain Analysis Small Anatomically-defined Areas Diet Drugs Early Life Adversity Lasting Changes Physiological Phenotypes Behavioural Phenotypes Metabolic Diseases Cardiovascular Diseases Mental Diseases Environmental Programming Gene-environment Dialogue Histone Modifications Mammals CpG Methylation Tissue-specific Methylation Cellular Heterogeneity Gene Expression And Methylation Correlation Epigenetic Programming Maternal Separation Paradigm Micropunches Isolation
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