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Journal / Neuroscience / Optimeret Analyse af DNA methylering og…
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JoVE Journal Neuroscience
Optimeret Analyse af DNA methylering og genekspression fra små, anatomisk definerede områder af hjernen
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Optimeret Analyse af DNA methylering og genekspression fra små, anatomisk definerede områder af hjernen

Article DOI: 10.3791/3938-v • 13:11 min • July 12th, 2012
July 12th, 2012 •
1, 1, 1, 1
1Max Planck Institute of Psychiatry

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En strømlinet arbejdsgang til at studere DNA methylering og genekspression ændringer ved tidlige-life stress vises. Gående ud fra moderens adskillelse af nyfødte mus og isolering af diskrete hjernevæv, vi repræsenterer en protokol til samtidig isolering af DNA og RNA fra hjernevæv patricer til efterfølgende bisulfit sekventering og RT-PCR-analyse.

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Neuroscience Genetik Fysiologi Epigenetik DNA methylering tidlige-livet stress moderen separation hydrogensulfit sekventering
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