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F1FO ATPase Vesicle Preparation and Technique for Performing Patch Clamp Recordings of Submitochondrial Vesicle Membranes
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
F1FO ATPase Vesicle Preparation and Technique for Performing Patch Clamp Recordings of Submitochondrial Vesicle Membranes
DOI:

08:21 min

May 04, 2013

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Chapters

  • 00:05Title
  • 02:00Brain Mitochondrial Isolation
  • 04:04Submitochondrial Vesicles Isolation
  • 05:13Electrophysiological Recording
  • 06:35Results: Patch Clamp Recordings of Submitochondrial Vesicle Membranes
  • 07:46Conclusion

Summary

Automatic Translation

A method to isolate submitochondrial vesicles enriched in F1FO ATP synthase complexes from rat brain is described. These vesicles allow the study of the activity of F1FO ATPase complex and its modulation using the technique of patch clamp recording.

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