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Coculture Analysis of Extracellular Protein Interactions Affecting Insulin Secretion by Pancreatic Beta Cells
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Coculture Analysis of Extracellular Protein Interactions Affecting Insulin Secretion by Pancreatic Beta Cells

Coculture Analysis of Extracellular Protein Interactions Affecting Insulin Secretion by Pancreatic Beta Cells

DOI:
10.3791/50365-v

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05:51 min

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June 15, 2013

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, ,
1Pediatric Diabetes Research Center,University of California, San Diego, 2Janssen Research & Development, 3Department of Medicine,University of California, San Diego

Chapters

  • 00:05Title
  • 01:15Transfection of HEK293 Layer
  • 01:50Co-culturing of INS-1 Beta Cells with HEK293 Cells
  • 03:03Glucose Stimulated Insulin Secretion
  • 04:21NL-X Engages in Transcellular Interactions that Increase Insulin Secretion
  • 05:25Conclusion

Summary

Automatic Translation

Automatic Translation

Transcellular protein interactions are important determinants of pancreatic beta-cell function. Detailed here is a method—adapted from a coculture model of synaptogenesis—for investigating how specific transmembrane proteins influence insulin secretion. Transfected HEK293 cells express proteins of interest; beta cells do not need to be transfected or otherwise directly perturbed.

Tags

Coculture AnalysisExtracellular Protein InteractionsInsulin SecretionPancreatic Beta CellsTransmembrane ProteinsTranscellular InteractionsBeta-cell FunctionHEK293 CellsGlucose-stimulated Insulin SecretionGene Expression

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