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Mouse Embryonic Development in a Serum-free Whole Embryo Culture System
JoVE Journal
Biology
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JoVE Journal Biology
Mouse Embryonic Development in a Serum-free Whole Embryo Culture System
DOI:

08:55 min

March 01, 2014

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Chapters

  • 00:05Title
  • 01:47Preparation
  • 02:41Collecting Embryos
  • 06:22Culturing Mouse Embryos
  • 07:37Cultured Mouse Embryos
  • 08:29Conclusion

Summary

Automatic Translation

Serum utilized in embryo cultures contains unknown components that can affect the outcome of experiments especially in studies involving signaling interactions. Here we utilized a serum-free oxygenated culture system and show that mid-gestation mouse embryos cultured for 16-40 hr exhibit morphological development comparable to embryos developing in utero.

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