Botulinum nörotoksin Işık Zinciri Modülatörlerin Belirlenmesi ve Karakterizasyonu için bir Yüksek verim uyumlu FRET tabanlı Platformu

Chemistry

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Caglič, D., Bompiani, K. M., Krutein, M. C., Čapek, P., Dickerson, T. J. A High-throughput-compatible FRET-based Platform for Identification and Characterization of Botulinum Neurotoxin Light Chain Modulators. J. Vis. Exp. (82), e50908, doi:10.3791/50908 (2013).

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Abstract

Materials

Name Company Catalog Number Comments
HEPES Teknova H1021
Tween-20 Fisher Scientific BP337-100
Methanol (HPLC-grade) Sigma-Aldrich 34860
Isopropanol (HPLC-grade) Sigma-Aldrich 650447
96-well Black assay plate Costar 3915
384-well Low-volume black assay plate Greiner 788076
SNAPtide FITC/Dabcyl substrate List Biological Laboratories 521 FRET-based BoNT/A LC substrate
Pin cleaning solution V&P Scientific VP 110
Lint-free blotting paper V&P Scientific VP 540DB
Biomek Seal and Sample Aluminum foil lids Beckman Coulter 538619

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References

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