Journal
/
Biology
/
Collection and Analysis of Arabidopsis Phloem Exudates Using the EDTA-facilitated Method
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Collection and Analysis of Arabidopsis Phloem Exudates Using the EDTA-facilitated Method

Collection and Analysis of Arabidopsis Phloem Exudates Using the EDTA-facilitated Method

DOI:
10.3791/51111-v

•

09:38 min

•

October 23, 2013

•

, ,
1Biochemistry and Molecular Biology,Michigan State Universtiy

Chapters

  • 00:05Title
  • 01:51Solution Preparation and Phloem Exudate Collection
  • 05:06Results: Analysis of Phloem Exudates
  • 08:17Conclusion

Summary

Automatic Translation

Automatic Translation

Knowledge of the composition of the phloem sap as well as the mechanism of its loading and long-distance transport is essential for the understanding of long-distance signaling in plant development and stress/pathogen response and of assimilate transport. This manuscript describes the collection of phloem exudates using the EDTA-facilitated method.

Tags

Phloem ExudateEDTA-facilitated MethodArabidopsisPhloem SapPhloem CompositionPhloem AnalysisPhloem TransportPlant DevelopmentStress ResponseMetabolite AnalysisProtein AnalysisRNA AnalysisPlant SpeciesTeaching Laboratory

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

A Simple Method for Imaging Arabidopsis Leaves Using Perfluorodecalin as an Infiltrative Imaging Medium

Cell Specific Analysis of Arabidopsis Leaves Using Fluorescence Activated Cell Sorting

An Efficient Method for Quantitative, Single-cell Analysis of Chromatin Modification and Nuclear Architecture in Whole-mount Ovules in Arabidopsis

Detection and Analysis of DNA Damage in Mouse Skeletal Muscle In Situ Using the TUNEL Method

Standardized Method for High-throughput Sterilization of Arabidopsis Seeds

A Versatile Method for Mounting Arabidopsis Leaves for Intravital Time-lapse Imaging

Two-layered Membrane Sandwich Method for Laodelphax striatellus Saliva Collection

High-throughput, Robust and Highly Time-flexible Method for Surface Sterilization of Arabidopsis Seeds

An Improved Chemotaxis Assay for the Rapid Identification of Rhizobacterial Chemoattractants in Root Exudates

Live Imaging of Arabidopsis Pollen Tube Reception and Double Fertilization Using the Semi-In Vitro Cum Septum Method

Read Article