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Preparation of Primary Myogenic Precursor Cell/Myoblast Cultures from Basal Vertebrate Lineages
JoVE Journal
Biology
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JoVE Journal Biology
Preparation of Primary Myogenic Precursor Cell/Myoblast Cultures from Basal Vertebrate Lineages
DOI:

07:51 min

April 30, 2014

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Chapters

  • 00:04Title
  • 01:18Preparation for Culture
  • 01:51Tissue Dissection
  • 03:05Mechanical Dissociation
  • 03:47Enzymatic Dissociation
  • 05:49Counting, Dilution, and Seeding of Cells
  • 06:23Results: Myoblasts and Myogenic Precursor Cells (MPCs) in Culture
  • 07:12Conclusion

Summary

Automatic Translation

In vitro culture systems have proven indispensible to our understanding of vertebrate myogenesis. However, much remains to be learned about nonmammalian skeletal muscle development and growth, particularly in basal taxa. An efficient and robust protocol for isolating the adult stem cells of this tissue, the myogenic precursor cells (MPCs), and maintaining their self-renewal, proliferation, and differentiation in a primary culture setting allows for the identification of conserved and divergent regulatory mechanisms throughout the vertebrate lineages.

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