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Monitoring Activation of the Antiviral Pattern Recognition Receptors RIG-I And PKR By Limited Protease Digestion and Native PAGE
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Monitoring Activation of the Antiviral Pattern Recognition Receptors RIG-I And PKR By Limited Protease Digestion and Native PAGE
DOI:

12:43 min

July 29, 2014

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Chapters

  • 00:05Title
  • 02:25Infection of A549 Cells with Rift Valley Fever Virus Clone 13 (Cl 13)
  • 04:17Preparation od Cell Lysates
  • 05:47Determination of Conformational Changes of Pattern Recognition Receptors
  • 08:40Analysis of Oligomeric States of Pattern Recognition Receptors
  • 09:46Results: RIG-I and PKR Assay
  • 12:00Conclusion

Summary

Automatic Translation

Innate defenses to virus infections are triggered by pattern recognition receptors (PRRs). The two cytoplasmic PRRs RIG-I and PKR bind to viral signature RNAs, change conformation, oligomerize, and activate antiviral signaling. Methods are described which allow to conveniently monitor the conformational switching and the oligomerization of these cytoplasmic PRRs.

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