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MicroRNA Expression Profiles of Human iPS Cells, Retinal Pigment Epithelium Derived From iPS, and Fetal Retinal Pigment Epithelium
JoVE Journal
Biology
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JoVE Journal Biology
MicroRNA Expression Profiles of Human iPS Cells, Retinal Pigment Epithelium Derived From iPS, and Fetal Retinal Pigment Epithelium
DOI:

10:19 min

June 24, 2014

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Chapters

  • 00:05Title
  • 01:41Differentiation of iPS Cells to iPS-Retinal Pigmented Epithelium
  • 03:32Collection of Cell Samples
  • 05:15Removal of Undifferentiated Cells from iPS-RPE Cells by Negative Selection
  • 06:36Total RNA Extraction
  • 07:08Pathway Analysis
  • 08:20Results: Differences in miRNA Expression Profiles of iPS-RPE as Compared to iPS and Fetal RPE
  • 09:21Conclusion

Summary

Automatic Translation

The microRNA (miRNA) profiles of human induced-pluripotent stem (iPS) cells, retinal pigment epithelium (RPE) derived from human induced-pluripotent stem (iPS) cells (iPS-RPE), and fetal RPE, were compared.

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