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Fundamental Technical Elements of Freeze-fracture/Freeze-etch in Biological Electron Microscopy
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Fundamental Technical Elements of Freeze-fracture/Freeze-etch in Biological Electron Microscopy

Fundamental Technical Elements of Freeze-fracture/Freeze-etch in Biological Electron Microscopy

DOI:
10.3791/51694-v

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11:17 min

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September 11, 2014

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1Department of Pediatrics, Center for Environmental Medicine, Asthma, and Lung Biology,The University of North Carolina at Chapel Hill

Chapters

  • 00:05Title
  • 02:15Freezing and Storage of Specimens in Advance of Freeze-fracture
  • 04:43Operation of the Freeze-fracture Instrument and Specimen Processing
  • 08:45Results: Structures Revealed by Freeze-fracture
  • 10:28Conclusion

Summary

Automatic Translation

Automatic Translation

Basic techniques and refinements of freeze-fracture processing of biological specimens and nanomaterials for examination by transmission electron microscopy are described. This technique is a preferred method for revealing ultrastructural features and specializations of biological membranes and for obtaining ultrastructural level dimensional and spatial data in materials sciences and nanotechnology products.

Tags

Freeze-fractureFreeze-etchElectron MicroscopyBiological SpecimensCell MembranesUltrarapid FreezingCryoprotective AgentsFracturingCarbon-platinum ReplicationThree-dimensional DetailCell UltrastructureCell BiologyNanotechnologyMaterials Science

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