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Journal / Immunology and Infection / Forenklede menneske Nøytrofile Ekstracellulære…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Forenklede menneske Nøytrofile Ekstracellulære Traps (NET) Isolasjon og håndtering
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Forenklede menneske Nøytrofile Ekstracellulære Traps (NET) Isolasjon og håndtering

Article DOI: 10.3791/52687-v • 09:43 min • April 16th, 2015
April 16th, 2015 •
*1, *1, 1, 1, 1
1LD MacLean Surgical Research Laboratories, Department of Surgery, McGill University
* These authors contributed equally

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I den etterfølgende protokoll beskriver vi en meget enkel måte å isolere Neutrofil Ekstracellulære feller (garn) fra humant fullblod ved å bruke lett tilgjengelige reagenser. Vi viser hvordan de isolerte NET kan brukes i en in vitro adhesjonsassayet med kreftceller.

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Immunologi Neutrophil nøytrofile ekstracellulære feller betennelser bakterier infeksjoner kreft metastase vedheft
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