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miRNA Expression Analyses in Prostate Cancer Clinical Tissues

Article DOI: 10.3791/53123-v • 11:29 min • September 8th, 2015
September 8th, 2015 •
1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1
1Department of Urology, Veterans Affairs Medical Center, San Francisco, University of California San Francisco

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Summary
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Here we describe a simplified protocol for microRNA (miRNA) expression analyses in archived Formalin-Fixed, Paraffin-Embedded (FFPE) or fresh frozen prostate cancer (PCa) clinical tissues employing quantitative real-time PCR (RT-PCR) and in situ hybridization (ISH).

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MiRNA Expression Analyses Prostate Cancer Clinical Tissues Biomarkers Disease Screening Diagnosis Prognosis Treatment MicroRNAs Alternate Biomarkers Accurate Detection Clinical Specimens Tumor Heterogeneity Sampling Errors Stromal Contamination Workflow Archived FFPE Fresh Frozen Quantitative Real-time PCR (RT-PCR) In Situ Hybridization (ISH) MiRNA Extraction Taqman-probe Based MiRNA RT-PCR ISH Analyses Locked Nucleic Acid (LNA) Based Probes Prostate Tissues Tissue Slides Tissue Microarrays (TMA)
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