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Journal / Neuroscience / Konstruktion av cellbaserade Neurotransmitter…
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JoVE Journal Neuroscience
Konstruktion av cellbaserade Neurotransmitter Fluorescerande Engineered Reportrar (CNiFERs) för optisk detektering av signalsubstanser
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Konstruktion av cellbaserade Neurotransmitter Fluorescerande Engineered Reportrar (CNiFERs) för optisk detektering av signalsubstanser

Article DOI: 10.3791/53290-v • 12:48 min • May 12th, 2016
May 12th, 2016 •
1, 2, 1, 2,3, 1
1Department of Neuroscience, Icahn School of Medicine at Mount Sinai, 2Department of Physics, University of California, San Diego, 3Section of Neurobiology, University of California, San Diego

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Vi presenterar ett protokoll för att skapa cellbaserad signalsubstans fluorescerande manipulerade reportrar (CNiFERs) för optisk detektering av volymetriska neurotransmittorfrisättning.

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Neurovetenskap optisk avbildning FSCV dialys volym överföring signalsubstanser neuropeptider TPLSM biosensor GPCR
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