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Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1
JoVE Journal
Biology
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JoVE Journal Biology
Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1
DOI:

11:02 min

May 27, 2016

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Chapters

  • 00:05Title
  • 01:04In Silico Prediction of Aternative First Exons of Bcrp1 Using BLAST Analysis of Mouse EST Database
  • 04:30Design of Reporter Constructs for Bcrp1 E1U, E1A, E1B, and E1C Promoters
  • 07:55Reporter Assay Methods
  • 09:03Results: Evaluation of Bcrp1 Alternative Promoter Function
  • 10:07Conclusion

Summary

Automatic Translation

With the murine ABC transporter Bcrp1 (Abcg2) as an example, in-silico protocols are presented to detect alternative promoter usage in genes expressed in mouse tissues, and to evaluate the functionality of the alternative promoters identified using reporter assays.

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