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JoVE Journal
Developmental Biology

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Isolation of Perivascular Multipotent Precursor Cell Populations from Human Cardiac Tissue
 

Isolation of Perivascular Multipotent Precursor Cell Populations from Human Cardiac Tissue

Article DOI: 10.3791/54252-v 08:15 min October 8th, 2016
October 8th, 2016

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Summary

Human cardiac tissue harbours multipotent perivascular precursor cell populations that may be suitable for myocardial regeneration. The technique described here allows for the simultaneous isolation and purification of two multipotent stromal cell populations associated with native blood vessels, i.e. CD146+CD34- pericytes and CD34+CD146- adventitial cells, from the human myocardium.

Tags

Isolation Perivascular Multipotent Precursor Cells Human Cardiac Tissue Cardiac Regeneration Heterogeneity Viability Adhesion Techniques Ischemic Heart Disease Cardiomyogenic Potential Myocardial Fibrosis Viable Cell Use Tissue Freshness Washing Medium Ventricular Myocardium Digestion Solution
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