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Fluorescence Activated Cell Sorting (FACS) and Gene Expression Analysis of Fos-expressing Neurons from Fresh and Frozen Rat Brain Tissue
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Fluorescence Activated Cell Sorting (FACS) and Gene Expression Analysis of Fos-expressing Neurons from Fresh and Frozen Rat Brain Tissue
DOI:

08:37 min

August 27, 2016

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Chapters

  • 00:05Title
  • 00:43Tissue Collection and Dissection
  • 02:17Cell Dissociation, Fixation and Permeabilization
  • 05:52Results: Representative Flow Cytometric and Gene Expression Analysis of Fos-expressing Neurons from Fresh and Frozen Brain Tissue
  • 07:16Conclusion

Summary

Automatic Translation

Here we present a Fluorescence Activated Cell Sorting (FACS) protocol to study molecular alterations in Fos-expressing neuronal ensembles from both fresh and frozen brain tissue. The use of frozen tissue allows FACS isolation of many brain areas over multiple sessions to maximize the use of valuable animal subjects.

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