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Imaging Cell Viability on Non-transparent Scaffolds — Using the Example of a Novel Knitted Titanium Implant
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Chapters
- 00:05Title
- 00:54Cleaning Knitted Titanium Scaffolds
- 02:27In Vitro Biocompatability Assay
- 03:30Live-dead Staining
- 04:33Resazurin Conversion Measurement
- 05:43Results: Cell Viability on the Scaffolds
- 06:43Conclusion
Here we present a fluorophore based imaging technique to detect cell viability on a non-transparent titanium scaffold as well as to detect glimpses of the scaffold impurities. This protocol troubleshoots the drawback of imaging cell-cell or cell-metal interactions on non-transparent scaffolds.
