Journal
/
/
Imaging Cell Viability on Non-transparent Scaffolds — Using the Example of a Novel Knitted Titanium Implant
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
Imaging Cell Viability on Non-transparent Scaffolds — Using the Example of a Novel Knitted Titanium Implant
DOI:

07:28 min

September 07, 2016

, , , , , , , ,

Chapters

  • 00:05Title
  • 00:54Cleaning Knitted Titanium Scaffolds
  • 02:27In Vitro Biocompatability Assay
  • 03:30Live-dead Staining
  • 04:33Resazurin Conversion Measurement
  • 05:43Results: Cell Viability on the Scaffolds
  • 06:43Conclusion

Summary

Automatic Translation

Here we present a fluorophore based imaging technique to detect cell viability on a non-transparent titanium scaffold as well as to detect glimpses of the scaffold impurities. This protocol troubleshoots the drawback of imaging cell-cell or cell-metal interactions on non-transparent scaffolds.

Related Videos

Read Article